Lab Protocols

Molecular Biology

  • Agarose Gel Electrophoresis
  • Bacterial Transformation
  • Creating Bacterial Glycerol Stocks
  • Diagnostic Restriction Digest
  • DNA Ligation
  • DNA Purification
  • DNA Quantification
  • How to Design a Primer
  • Inoculating a Liquid Bacterial Culture
  • Making LB Agar Plates
  • Over-Agar Antibiotic Plating
  • Polymerase Chain Reaction (PCR)
  • Purifying DNA from an Agarose gel
  • Restriction Digests
  • Sequence Analysis
  • Streaking Bacteria
  • Cloning by PCR
  • Gibson Assembly
  • Ligation Independent Cloning (LIC)
  • Modification by Annealed Oligo Cloning
  • Restriction Cloning
  • CIS Display Selection
  • Preparation of PEI transfection solution

    • Virus Production and Purification

  • AAV Production in HEK293 Cells
  • AAV Purification by Iodixanol Gradient Ultracentrifugation
  • AAV Titration by qPCR Using SYBR Green Technology
  • Colony Formation Titering Assay for Lentivirus
  • Fluorescence Titering Assay for Lentivirus
  • Lentivirus Production
  • Concentrate Lentivirus with PEG8000 precipitation

    • Cell Culture

  • General Transfection
  • Generating Stable Cell Lines with Lentivirus
  • Isolating a Monoclonal Cell Population by Limiting Dilution
  • Satellite Cell Isolation Kit (mouse)
  • Primary myoblast culture
  • Protein production and extraction in E. Coli
  • Phage display

    • Miscellaneous

    Biochemistry

  • SDS-PAGE Gradient Gel (3-15%)
  • Co-IP

    • Immunofluorescence

  • Evans Blue Dye uptake
  • Isolated FDB muscle fibers for damage-repair assay
  • rabbit cardiomyocyte isolation